Identification of conditions for transfection of sgRNAs

Two-hundred and forty conditions are tested to define optimal transfection format, using eight different transfection reagents and varied amounts of a suitable control sgRNA.

$1,500

Optimization of assay conditions for high throughput screening

Cell density, main readout, and automation conditions are established for high throughput screening.

$1,500

High throughput screening (HTS) of whole genome sgRNA CRISPR library (human or mouse)

High throughput screening of 384-well microplates(68 plates encompassing a complete sgRNA library), including automation, data collection and analysis, is performed.

$6,000

Validation of sgRNA-mediated gene knockout of selected hits from HTS

Genomic deletions are confirmed using PCR and sequencing of PCR products of selected hits

$1,500