Multiplexing Protein Analysis Core

CORE OVERVIEW

The Multiplexing Protein Analysis Laboratory uses LC-tandem MS with selected reaction monitoring (SRM) and LC-high resolution accurate mass selected ion monitoring (SIM) to measure protein abundance and isotope enrichment. The protein abundance measurements are unique because proteins are carefully selected and targeted for quantification. Targeting is achieved by designing methods to measure the abundance of several peptides. Adding deuterium oxide during the experimental period allows the determination of the synthesis rates of targeted proteins. An investigator using the core can then determine dynamic regulation of individual proteins.